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ÀÌ ¹æ¹ýÀº ¾çÀ̿ ¼º ºÐÀÚ¸¦ ÄÚÆÃÇÑ ÀÚ¼º ³ª³ëÀÔÀÚ¿Í DNA¿ÍÀÇ °áÇÕÀ»
ÀÌ¿ëÇÑ °ÍÀÔ´Ï´Ù. MagnetofectionÀº À¯ÀüÀÚ º¤ÅÍ¿¡ ÀÚ·ÂÀ» ÁÖ¾î
¸ñÀûÇÏ´Â ¼¼Æ÷ ¾ÈÀ¸·Î µé¾î°¥ ¼ö ÀÖµµ·Ï ÇÕ´Ï´Ù. ÀÌ·¯ÇÑ ¹æ¹ýÀ¸·Î ¼ö ºÐ
¾È¿¡ ³Ö¾îÁØ ¸ðµç º¤ÅͰ¡ ¼¼Æ÷ ¾ÈÀ¸·Î µé¾î°¡ 100%ÀÇ ¼¼Æ÷°¡ ÃæºÐÇÑ ¾çÀÇ
º¤Å͸¦ Áö´Ï°Ô µË´Ï´Ù. ÀÔÁõµÇ°í ÀÎÁ¤ ¹ÞÀº ÀÚ¼ºÀ» ÀÌ¿ëÇÑ targeting±â¼úÀ»
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»ç¿ëÇÒ ¼ö ÀÖÀ¸¸ç, ÀÀ¿ëÀÌ ´Ù¾çÇϰí, ¼¼Æ÷¿¡ ´ëÇØ ¹«µ¶Çϸç, ±×¸®°í °æÁ¦ÀûÀÎ
¹æ¹ýÀ̶ó°í ÇÒ ¼ö ÀÖ½À´Ï´Ù.
´Ü¼øÇÑ ¹æ¹ý ±×¸®°í
½Å¼ÓÇÔ
°íÈ¿À²
ÀÚ¼ºÀÔÀÚ¸¦ ¼¼Æ÷ ³»¿¡ ÁýÁß½ÃÅ´À¸·Î½á, ¾ÆÁÖ ÀûÀº ¾çÀÇ DNA ³ªvector·Îµµ
transfection È¿À²À» ±Ø´ëÈ ÇÒ ¼ö ÀÖ½À´Ï´Ù.
Confluent Primary
Human Keratinocytes

Standard Transfection
Magnetofection
Commercial Reagent L
(PolyMag)
¾î´À ¼¼Æ÷¿¡µµ »ç¿ëÇÒ
¼ö ÀÖ½À´Ï´Ù.
primary cells¿¡ transfection½ÃŰ´Â ¹æ¹ý Áß °¡Àå
ÀÌ»óÀûÀÎ ¹æ¹ýÀ̶ó°í ÇÒ ¼ö ÀÖ½À´Ï´Ù.
Primary Cells
Chondrocytes (Photo)
Peripherical Blood Lymphocytes
Aortic Endothelial Cells
Human Fibroblasts
Nasal & Pulmonary Epithelium...
¼¼Æ÷¿¡ transfection½ÃŰ´Â ¹æ¹ý Áß¿¡¼ °¡Àå È¿À²ÀûÀÎ ¹æ¹ýÀÔ´Ï´Ù.
Cell Lines
293
MCF-7
HeLa
Hep G2
CHO-K1
´Ù¾ç¼º
OZ Biosciences´Â µÎ °¡Áö Á¾·ùÀÇ ½Ã¾àÀ» Á¦°øÇØ µå¸®°í ÀÖ½À´Ï´Ù.
PolyMag
CT-26 Colon Carcinoma
Standard Transfection Magnetofection
PolyMag ´Â ³ôÀº È¿À²·Î ÇÙ»êÀ» ¼¼Æ÷ ³»¿¡ Àü´ÞÇÏ´Â Á¦Ç°ÀÔ´Ï´Ù.
naked DNA, plasmid DNA, antisense oligonucleotides
and siRNA µîÀÇ transfectionÀ» ¼º°øÀûÀ¸·Î µµ¿Í µå¸³´Ï´Ù.
CombiMag

NIH 3T3 Cells lacking CAR receptor infected
with an Adenovirus (coding for LacZ) +/-
CombiMag with (right) and without (left)
magnetic field
CombiMag ´Â ¸ðµç Á¾·ùÀÇ º¤ÅÍ¿¡ Àû¿ëÇÒ ¼ö ÀÖ´Â À¯ÀÏÇÑ ¹æ¹ýÀÔ´Ï´Ù.
´ÙÀ½°ú °°ÀÌ º¹ÇÕÀûÀ¸·Î »ç¿ëÇÏ½Ç ¼ö ÀÖ½À´Ï´Ù
o ¾î¶² »ó¾÷ÀûÀ¸·Î ÀÌ¿ë °¡´ÉÇÑ transfection ½Ã¾àÀ̳ª ¸ðµç Á¾·ùÀÇ
ÇÙ»ê°ú ÇÔ²² »ç¿ëÇÒ ¼ö ÀÖ½À´Ï´Ù. Áö±Ý±îÁö »ç¿ëÇϽôø ¹æ¹ýÀ» ¹Ù²Ü ÇÊ¿ä
¾øÀÌ ±× ¹æ¹ý¿¡ ÷°¡ÇÏ¿© transfectionÈ¿À²À» ³î¶ø°Ô Áõ°¡½Ãų ¼ö
ÀÖ½À´Ï´Ù.
o ¹ÙÀÌ·¯½º¿Í ÇÔ²² »ç¿ëÇÏ¿© ¼÷ÁÖÀÇ ¹ÙÀÌ·¯½º vvector¿¡ ´ëÇÑ Ä£È¼ºÀ»
³ÐÈ÷°í(adenoviruses), ¹ÙÀÌ·¯½ºÀÇ °¨¿°È¿À² (retroviruses)À»
°³¼±ÇÒ ¼ö ÀÖ½À´Ï´Ù.
Standard Transfection Magnetofection
(CombiMag)
¼¼Æ÷¿¡°Ôµµ ¿¬±¸ÀÚ¿¡°Ôµµ
¸ðµÎ ¾È¶ôÇÔÀ» µå¸³´Ï´Ù.
¹«µ¶¼º
´õ ÀÌ»ó ¼¼Æ÷¿¡°Ô Àü±âÀûÀÎ Ãæ°ÝÀ» ÁÙ Çʿ䰡 ¾ø½À´Ï´Ù.

°æÁ¦¼º
o °ªºñ½Ñ ÀåÄ¡°¡ ÇÊ¿ä ¾ø½À´Ï´Ù.
o Àç·á¸¦ ¾Æ³¥ ¼ö ÀÖ½À´Ï´Ù.¾ÆÁÖ ÀûÀº ¾çÀÇ DNA·Îµµ ÃæºÐÇÕ´Ï´Ù.
o ºñ¿ëÀ» Àý¾àÇÒ ¼ö ÀÖ½À´Ï´Ù: ½Ã¾à »ç¿ë·®ÀÌ Àû½À´Ï´Ù.
o ºñ¿ë´ëºñ ÃÖ°íÀÇ È¿À²
|
Catalog
Number
|
Product
|
Volume
(¥ìL)
|
Size
(# of Transf./¥ìg of DNA)
|
#
of Transf./96
well plates
|
|
KC-30296
|
Starting
Kit©ö
|
2
X 100
|
2
X 100
|
2
X 1000
|
|
KM-30200
|
Selection
Kit©÷
|
2
X 100
|
2
X 100
|
2
X 1000
|
|
MF-10096
|
Magnetic
Plate
|
-
|
-
|
-
|
|
PM-10100
|
PolyMag
100
|
100
|
100
|
1000
|
|
PM-10200
|
PolyMag
200
|
200
|
200
|
2000
|
|
PM-11000
|
PolyMag
1000
|
1000
|
1000
|
10000
|
|
CM-20100
|
CombiMag
100
|
100
|
100
|
1000
|
|
CM-20200
|
CombiMag
200
|
200
|
200
|
2000
|
|
CM-21000
|
CombiMag
1000
|
1000
|
1000
|
10000
|
1. Contain : PM10100 + CM20100 + magnetic
plate
2. Contain : PM10100 + CM20100
|
|
©¬-galactosidase Assay Kit |
|
|
TransfectionµÈ ¼¼Æ÷¿¡¼ ©¬-galactosidase
ÀÇ ¹ßÇö Á¤µµ¸¦ Á¤·®ÀûÀ¸·Î ÃøÁ¤ÇÒ ¼ö Àִ ŰƮ·Î¼, ¹Î°¨ÇÑ ±âÁúÀÎ,
CPRG and ONPG¸¦ »ç¿ëÇÏ¿© ÃøÁ¤Çϴ ŰƮÀÔ´Ï´Ù. |
|
|
X-Gal
Staining Kit |
|
|
in situ ©¬-galactosidase
¿°»öÀ» ¹ÙÅÁÀ¸·Î transfection efficiency À» °áÁ¤ÇÏ´Â
µ¥ »ç¿ëÇÏ´Â Ready-to-use assay system ÀÔ´Ï´Ù. |
|