OZ BIOSCIENCESȍ

ÀÌÁ¦ transfection¿¡ ´ëÇÑ °ÆÁ¤Àº ¾È Çϼŵµ µË´Ï´Ù.

»õ·Î¿î Transfection ±â¼ú !!

ÀÌ ¹æ¹ýÀº ¾çÀ̿ ¼º ºÐÀÚ¸¦ ÄÚÆÃÇÑ ÀÚ¼º ³ª³ëÀÔÀÚ¿Í DNA¿ÍÀÇ °áÇÕÀ» ÀÌ¿ëÇÑ °ÍÀÔ´Ï´Ù. MagnetofectionÀº À¯ÀüÀÚ º¤ÅÍ¿¡ ÀÚ·ÂÀ» ÁÖ¾î ¸ñÀûÇÏ´Â ¼¼Æ÷ ¾ÈÀ¸·Î µé¾î°¥ ¼ö ÀÖµµ·Ï ÇÕ´Ï´Ù. ÀÌ·¯ÇÑ ¹æ¹ýÀ¸·Î ¼ö ºÐ ¾È¿¡ ³Ö¾îÁØ ¸ðµç º¤ÅͰ¡ ¼¼Æ÷ ¾ÈÀ¸·Î µé¾î°¡ 100%ÀÇ ¼¼Æ÷°¡ ÃæºÐÇÑ ¾çÀÇ º¤Å͸¦ Áö´Ï°Ô µË´Ï´Ù. ÀÔÁõµÇ°í ÀÎÁ¤ ¹ÞÀº ÀÚ¼ºÀ» ÀÌ¿ëÇÑ targeting±â¼úÀ» ÀÌ¿ëÇÑ ¹æ¹ýÀ¸·Î, ÀÌ ±â¼úÀº ´Ü¼øÇϰí, ºü¸£¸ç, ³ôÀº È¿À²À» Áö´Ï°í, ¹ü¿ëÀ¸·Î »ç¿ëÇÒ ¼ö ÀÖÀ¸¸ç, ÀÀ¿ëÀÌ ´Ù¾çÇϰí, ¼¼Æ÷¿¡ ´ëÇØ ¹«µ¶Çϸç, ±×¸®°í °æÁ¦ÀûÀÎ ¹æ¹ýÀ̶ó°í ÇÒ ¼ö ÀÖ½À´Ï´Ù.

´Ü¼øÇÑ ¹æ¹ý ±×¸®°í ½Å¼ÓÇÔ


°íÈ¿À²

ÀÚ¼ºÀÔÀÚ¸¦ ¼¼Æ÷ ³»¿¡ ÁýÁß½ÃÅ´À¸·Î½á, ¾ÆÁÖ ÀûÀº ¾çÀÇ DNA ³ªvector·Îµµ transfection È¿À²À» ±Ø´ëÈ­ ÇÒ ¼ö ÀÖ½À´Ï´Ù.

Confluent Primary Human Keratinocytes



Standard Transfection             Magnetofection
Commercial Reagent L              (PolyMag)


¾î´À ¼¼Æ÷¿¡µµ »ç¿ëÇÒ ¼ö ÀÖ½À´Ï´Ù.

primary cells¿¡ transfection½ÃŰ´Â ¹æ¹ý Áß °¡Àå ÀÌ»óÀûÀÎ ¹æ¹ýÀ̶ó°í ÇÒ ¼ö ÀÖ½À´Ï´Ù.



Primary Cells
Chondrocytes (Photo)
Peripherical Blood Lymphocytes
Aortic Endothelial Cells
Human Fibroblasts
Nasal & Pulmonary Epithelium...

¼¼Æ÷¿¡ transfection½ÃŰ´Â ¹æ¹ý Áß¿¡¼­ °¡Àå È¿À²ÀûÀÎ ¹æ¹ýÀÔ´Ï´Ù.



Cell Lines
293
MCF-7
HeLa
Hep G2
CHO-K1

´Ù¾ç¼º

OZ Biosciences´Â µÎ °¡Áö Á¾·ùÀÇ ½Ã¾àÀ» Á¦°øÇØ µå¸®°í ÀÖ½À´Ï´Ù.

PolyMag

CT-26 Colon Carcinoma

Standard Transfection               Magnetofection

PolyMag ´Â ³ôÀº È¿À²·Î ÇÙ»êÀ» ¼¼Æ÷ ³»¿¡ Àü´ÞÇÏ´Â Á¦Ç°ÀÔ´Ï´Ù. naked DNA, plasmid DNA, antisense oligonucleotides and siRNA µîÀÇ transfectionÀ» ¼º°øÀûÀ¸·Î µµ¿Í µå¸³´Ï´Ù.

CombiMag



NIH 3T3 Cells lacking CAR receptor infected with an Adenovirus (coding for LacZ) +/- CombiMag with (right) and without (left) magnetic field

CombiMag ´Â ¸ðµç Á¾·ùÀÇ º¤ÅÍ¿¡ Àû¿ëÇÒ ¼ö ÀÖ´Â À¯ÀÏÇÑ ¹æ¹ýÀÔ´Ï´Ù. ´ÙÀ½°ú °°ÀÌ º¹ÇÕÀûÀ¸·Î »ç¿ëÇÏ½Ç ¼ö ÀÖ½À´Ï´Ù

o ¾î¶² »ó¾÷ÀûÀ¸·Î ÀÌ¿ë °¡´ÉÇÑ transfection ½Ã¾àÀ̳ª ¸ðµç Á¾·ùÀÇ ÇÙ»ê°ú ÇÔ²² »ç¿ëÇÒ ¼ö ÀÖ½À´Ï´Ù. Áö±Ý±îÁö »ç¿ëÇϽôø ¹æ¹ýÀ» ¹Ù²Ü ÇÊ¿ä ¾øÀÌ ±× ¹æ¹ý¿¡ ÷°¡ÇÏ¿© transfectionÈ¿À²À» ³î¶ø°Ô Áõ°¡½Ãų ¼ö ÀÖ½À´Ï´Ù.

o ¹ÙÀÌ·¯½º¿Í ÇÔ²² »ç¿ëÇÏ¿© ¼÷ÁÖÀÇ ¹ÙÀÌ·¯½º vvector¿¡ ´ëÇÑ Ä£È­¼ºÀ» ³ÐÈ÷°í(adenoviruses), ¹ÙÀÌ·¯½ºÀÇ °¨¿°È¿À² (retroviruses)À» °³¼±ÇÒ ¼ö ÀÖ½À´Ï´Ù.


Standard Transfection            Magnetofection
                                 (CombiMag)

¼¼Æ÷¿¡°Ôµµ ¿¬±¸ÀÚ¿¡°Ôµµ ¸ðµÎ ¾È¶ôÇÔÀ» µå¸³´Ï´Ù.

¹«µ¶¼º


´õ ÀÌ»ó ¼¼Æ÷¿¡°Ô Àü±âÀûÀÎ Ãæ°ÝÀ» ÁÙ Çʿ䰡 ¾ø½À´Ï´Ù.



°æÁ¦¼º

o °ªºñ½Ñ ÀåÄ¡°¡ ÇÊ¿ä ¾ø½À´Ï´Ù.
o Àç·á¸¦ ¾Æ³¥ ¼ö ÀÖ½À´Ï´Ù.¾ÆÁÖ ÀûÀº ¾çÀÇ DNA·Îµµ ÃæºÐÇÕ´Ï´Ù.
o ºñ¿ëÀ» Àý¾àÇÒ ¼ö ÀÖ½À´Ï´Ù: ½Ã¾à »ç¿ë·®ÀÌ Àû½À´Ï´Ù.
o ºñ¿ë´ëºñ ÃÖ°íÀÇ È¿À²


Catalog Number
Product
Volume
(¥ìL)
Size
(# of Transf./¥ìg of DNA)
# of Transf./96
well plates
KC-30296
 Starting Kit©ö
2 X 100
2 X 100
2 X 1000
KM-30200
 Selection Kit©÷
2 X 100
2 X 100
2 X 1000
MF-10096
 Magnetic Plate
-
-
-
PM-10100
 PolyMag 100
100
100
1000
PM-10200
 PolyMag 200
200
200
2000
PM-11000
 PolyMag 1000
1000
1000
10000
CM-20100
 CombiMag 100
100
100
1000
CM-20200
 CombiMag 200
200
200
2000
CM-21000
 CombiMag 1000
1000
1000
10000

1. Contain : PM10100 + CM20100 + magnetic plate
2. Contain : PM10100 + CM20100

 ©¬-galactosidase Assay Kit
TransfectionµÈ ¼¼Æ÷¿¡¼­ ©¬-galactosidase ÀÇ ¹ßÇö Á¤µµ¸¦ Á¤·®ÀûÀ¸·Î ÃøÁ¤ÇÒ ¼ö Àִ ŰƮ·Î¼­, ¹Î°¨ÇÑ ±âÁúÀÎ, CPRG and ONPG¸¦ »ç¿ëÇÏ¿© ÃøÁ¤Çϴ ŰƮÀÔ´Ï´Ù.
 X-Gal Staining Kit
in situ ©¬-galactosidase ¿°»öÀ» ¹ÙÅÁÀ¸·Î transfection efficiency À» °áÁ¤ÇÏ´Â µ¥ »ç¿ëÇÏ´Â Ready-to-use assay system ÀÔ´Ï´Ù.